Fig. 1. Generation of the AMM/I cell line via TALEN. (A) Schematic diagram of the donor vector carrying IL-10 and donor plasmid DNA targeting locus (AAVS1 site). The expression cassette containing the PGK promoter-driven IL-10 and EF1α promoter-driven GFP-T2A-puromycin were inserted into the AAVS1 site by homology-directed repair. The locations of primers for junction detection are indicated (primers F and R). HA-L, left homology arm; HA-R, right homology arm; PGK, phosphoglycerate kinase promoter; EF1α, elongation factor-1 alpha promoter; Puro, puromycin. (B) Identification of the correct insertion of the donor plasmid into the AAVS1 locus by junction PCR. (C) Representative picture of GFP expressing the transfected AMM/I line. Transfected cells were purified by puromycin and fluorescence-activated cell sorting. Bars=200µm. (D) Quantification of mRNA expression levels in the AMM/I cell line. **p<0.01; n = 4 per group.